Precision-cut bovine udder slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis

Abstract Background The aim of this study was to establish precision-cut bovine udder slices (PCBUS) as an in-vitro-model investigate pathophysiological processes in the early phase mastitis order have possibility new therapeutic approaches for treatment such inflammation later studies. Furthermore, model should contribute substitute in-vivo-experiments. Bovine is one most common and costly infectious diseases dairy industry, which largely associated with use antimicrobial agents. Given problem resistance, it essential step up research into bacterial diseases. Thus, transfer tissue enables broad area can also be used address issues basic or characterisation complex processes. Results A stimulation LPS, PGN combination both substances (LPS:PGN) demonstrates ability PCBUS react a significant secretion IL-1ß, TNF-? PGE 2 . Conclusion represent instrument investigating pharmacological interactions tissue, useful studies on questions understanding infection inflammation.